The emergence of multidrug-resistant bacteria in animals and their products has highlighted the considerable value of the potential transfer of resistance to the human population via the food chain. The objective of this work was to study the antibiotic resistance profile of staphylococci of human and animal origin and to compare the two profiles in order to define an effective therapeutic and preventive strategy. This work was carried out on a set of 97 strains of staphylococci isolated from animal and human biological liquids. The isolation and identification was done by conventional methods. The determination of the minimum inhibitory concentrations (MIC) was studied by the reference method (dilution in agar medium) and VITEK 2® with respect to eight (8) antibiotic molecules. The results were interpreted according to the ACFSM criteria (2001). The results showed higher resistance of human bacteria to penicillin (72.22% vs. 30.23%) erythromycin (50% vs. 18.6%) and gentamicin (33.33% vs. 9.3%) compared to animal bacteria. On the other hand, the resistance to fosfomycin of staphylococci of animal origin was higher (60.46%) compared to human strains (38.89%). Indeed, it seems that vancomycin and rifampicin are the antibiotics of choice against these pathogenic strains in veterinary and human medicine.