Overproduction of oxygen free radicals (ROS) and oxidative stress during reperfusion period plays a critical role in pathophysiology of cerebral ischemia-reperfusion injury. Since the antioxidant properties of fullerene nanoparticles has been extensively demonstrated, we assessed the possible protective effects of water-soluble fullerene derivatives (C60(OH)18-22) on infarct volume and edema after middle cerebral artery occlusion in rat.
Twenty four rats were randomly divided into three groups (each group; n=8): sham, control ischemia (IR) and ischemic treatment groups. Cerebral ischemia was induced by 90 min middle cerebral artery occlusion (MCAO) followed by 24 hours reperfusion. Fullerene nanoparticles were given orally at dose of 1mg/kg immediately after termination of MCAO and the beginning of reperfusion period. The brains were processed for histopathological assessment and quantitation of the infarct volume (TTC staining method). Finally, the brain hemispheres were weighed as an index of brain edema.
There was no infarction and edema in right hemispheres of sham rats. MCAO induced brain infarction both in cortex (261Â±23mm3) and subcortex (138Â±23mm3) and also right hemispheres weights (23%) of control ischemic rats. Administration of fullerene reduced the infarction both in cortex (49Â±26mm3) and subcortex (43Â±21mm3) and also the value of right hemisphere weight (20%) of ischemic treatment rats. Additionally in histopathological assessment, the perivascular areas decreased in ischemic treatment rats.
The findings of present study are revealing the protective effects of water-soluble fullerene derivatives against reperfusion-induced injury such as infarction and edema after middle cerebral artery occlusion in rat.