To evaluate the anti-inflammatory properties of the fractions of Commelina ascendens in rats. Egg albumin induced paw oedema and cotton pellet induced granuloma in rats were used to determine the anti-inflammatory activities of n-hexane (HF), ethylacetate (EF) and butanol (BF) fractions of Commelina ascendens. BF (200 mg/kg) significantly inhibited (p<0.05) oedema induced by egg albumin by producing 3.22 % protection at 4 h post-treatment. EF, HF and BF significantly (p<0.05) decreased the granuloma tissue caused by cotton pellet. The phytochemical screening of HF, EF and BF revealed the presence of saponin, tannins, flavonoids, steroids, carbohydrates. The extract did not show any sign of toxicity up to 5000 mg/kg body weight of mice. The results obtained justified the use of this plant in traditional medicine for the treatment of inflammation and may provide a basis for the development of anti-inflammatory agents.