It has been recently reported that cannabinoid and GABA systems in the nucleus accumbensÂ (NAc) are involved in anxiety-related behaviors. Thus the purpose of the present study is to investigateÂ the involvement of ACPA interaction with GABA-ergic of the NAc shell in anxiolytic-like behaviors in Wistar male rat. The elevated plus maze apparatus has been used to testÂ parameters of anxiety-like behaviors. The data demonstrated that bilateral injection of GABAÂ (A) receptor agonist (Muscimol 0.4 Î¼g/Î¼l) increased % OAT. Furthermore, injection of GABAÂ (A) receptor antagonist (Bicuculline 0.9Î¼g/Î¼l) increased locomotor activity. The data indicatedÂ anxiolytic-like behaviors caused by the Muscimol injection into the NAc shell. Moreover, bilateralÂ injection of ACPA (CB1 -selective agonists; 0.025Î¼g/Î¼l) into the NAc shell inducedÂ anxiogenic-like behaviors. The final results showed that intra-NAc shell injection of subthresholdÂ dose of Muscimol (0.2 Î¼g/Î¼l) attenuated anxiogenic-like behaviors by higher dose (0.025Â Î¼g/Î¼l) of ACPA in the NAc shell. In addition, intra-NAc shell injection of subthreshold dose ofÂ Bicuculline (0.6 Î¼g/Î¼l) did not alter anxiogenic response by ACPA administration in the NAcÂ shell. The results suggested that the activation of the NAc shell GABA (A) receptor attenuatedÂ the activity of cannabinoid system in the NAc shell.