Increased number of population with renal damage is attributed to sedentary life styleÂ and consumption of high sugar diets especially fructose. The purpose of present study is toÂ investigate the renoprotective activity of aqueous extract of Phyllanthus amarus (PAAEt)Â against high fructose diet (HFD) induced renal damage (RD) in Wistar rats. High fructoseÂ diet (66% fructose) alone and in combination with PAAEt (200 mg/kg/body wt/day) wasÂ given simultaneously to group-F and group-F+T rats respectively for a period of 60 days.Â Functional markers of renal tissue such as, urea, uric acid and creatinine levels in plasmaÂ were quantified on initial and final day of the experiment. Activities of transaminases, gluconeogenicÂ enzymes such as, glucose-6-phosphatase (G-6-Pase), and fructose-1, 6-Â bisphosphatase (F-1,6-BPase) and polyolpathway enzymes such as, aldose reductase andÂ sorbitol dehydrogenase were assayed in kidney. Oxidative stress (OS) markers such as, lipidÂ peroxidation and protein oxidation and antioxidants status were measured in renal tissue.Â Renal histopathological changes were also examined. Co-treatment with PAAEt to group-F+T prevented the rise in the levels of functional markers and elevated activities of transaminases,Â gluconeogenic and polyolpathway enzymes of group-F (P < 0.05). OS developed inÂ group-F by elevated stress markers and depletion of antioxidants were prevented in group-F+T. The observed histological changes in group-F were protected in group-F+T. Group-C+T showed no histological changes with group-C. Thus, PAAEt effectively alleviated fructoseÂ diet induced RD which may be due to its antioxidant activity. Hence this plant could beÂ used as an adjuvant therapy for the prevention and/or management of HFD induced RD.