Interplay between lipo-polysaccahride induced oxidative stress and amyloid beta deposition in Alzheimer’s disease and treatment with glycyrrhizin

Keywords

Neurodegenerative disease
Glycyyhizin
Lipo-polysaccharide
Y-maze test
Oxida

How to Cite

Abdelghany, R. M., Sharaf, N. M., El Dine, N. S., & Mahran, L. G. (2014). Interplay between lipo-polysaccahride induced oxidative stress and amyloid beta deposition in Alzheimer’s disease and treatment with glycyrrhizin. Journal of Experimental and Applied Animal Sciences, 1(2), 212–228. https://doi.org/10.20454/jeaas.2014.812

Abstract

Oxidative stress (OS) plays a crucial role in the pathogenesis of neurodegenerative disease like Alzheimer’s disease (AD). Glycyrrhizin (Gly) is one of the components of licorice roots possesses potent anti-oxidant, anti inflammatory and immune-modulatory properties. Lipopolysaccharide (LPS), which is the main constituent of the cell wall of Gramnegative bacteria, causes massive oxidative damage in the brain upon systemic administration. The aim of this study is to investigate the neuro-protective effect of Gly against OS induced neuronal damage as a result of LPS administration. 72 mice were used; Gp I: Saline, Gp II: LPS, Gp III: Deprenyl (Dep) (positive control) and Gps IV, V, VI: Gly (5, 10 and 20 mg/kg) respectively. Y-maze test and Open field test were performed to assess spatial memory and to determine the motor activity in mice respectively. Lipid peroxidation (by measuring thiobarbituric acid reactive species; TBARS), reduced glutathione (GSH) levels, and superoxide dismutase (SOD) enzyme activity were determined biochemically in brain homogenates. Amyloid beta (Aβ) deposition was evaluated using immune-histochemistry in dentate gyrus of the hippocampus and the cerebral cortex. Results showed that LPS significantly increased TBARS and suppressed GSH level and SOD enzyme activity in addition to altering spatial memory and motor activity. Dep and the three doses of Gly significantly inhibited OS induced Aβ deposition and significantly improved both spatial memory and motor activity. 20 mg/kg Gly was proven to produce maximum protection against LPS induced OS. Conclusively, these findings may advocate the protective effect of Gly against AD through its antioxidant effect.

https://doi.org/10.20454/jeaas.2014.812

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