Protective role of aqueous extract of Moringa oleifera leaf against oxidative stress and histological alterations in testes of rats under insulin deficient condition
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D, S. M., B, S. R., B, R., B, R., & D, S. (2014). Protective role of aqueous extract of Moringa oleifera leaf against oxidative stress and histological alterations in testes of rats under insulin deficient condition. Journal of Experimental and Applied Animal Sciences, 1(2), 241–252. https://doi.org/10.20454/jeaas.2014.856

Abstract

The current study investigates the effect of Aqueous Extract of Moringa oleifera (AEMO)  leaf on the oxidative stress, antioxidant status and histopathological changes in testes of rats under insulin deficient condition. Male Wistar rats were used in this study. Diabetes (insulin deficient) was induced in rats by a single intraperitoneal injection of Streptozotocin. AEMO was administered at a dose of 200 mg/kg body weight for a period of 60 days. At the end of the experimental period, the rats were sacrificed by cervical dislocation. Relative organ weight was calculated. Oxidative stress was assessed by the extent of lipid peroxidation (LPO) and the levels of reduced Glutathione (GSH) in testes. The status of antioxidant enzymes was estimated by determing the activities of Glutathione Reductase (GR), Glutathione- S-Transferase (GST), Sorbitol dehydrogenase (SDH),[Catalase (CAT) and Superoxide dismutase (SOD). Histological changes in testes were studied. Statistical significance of the results was evaluated by Duncan’s Multiple Range Test. In this study, diabetic rats showed significant decrease in

absolute and relative weights of testes, increased LPO and decreased levels
of GSH.
The activities of antioxidantm enzymes- GR, GST, SOD, CAT were reduced. In contrast, the activity of SDH, a polyolpathway enzyme, increased. Histological picture of testes of D group showed pathological changes. However, administration of AEMO showed beneficial effect which was reflected by reduced oxidative stress, enhanced activities of antioxidant enzymes in testes. AEMO also protected the testes against the histological changes induced by oxidative stress under insulin deficient condition. The study revealed that AEMO ameliorated oxidative stress, exhibited antioxidant potential, and played a role in the protection of testicular structural integrity under insulin deficient condition.

https://doi.org/10.20454/jeaas.2014.856
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